PRIMERS DESIGNING & IN-SILICO PCR

PRIMERS DESIGNING & IN-SILICO PCR

EGP 0

Science Shake experts design the primers using web-based tools like “Primer3” and “Web Primer”. For designing the primer, DNA template sequence is extracted from any of the available sequence databases, e.g., RefSeq database. The in-silico PCR validation can be then carried out using BLAST tool and Gene Runner software, which check their efficiency and specificity. Thereafter, the designed primers can be validated and synthesized in the wet lab for use in the amplification of concerned gene/DNA fragment.

Initial response should be received within 3-5 days.